Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Effects of the long and short isoforms of TIPE3 on the growth and metastasis of gastric cancer.

doi: 10.1016/j.biopha.2020.109853

Figure Lengend Snippet: Fig. 4. Long TIPE3 and short TIPE3 promoted the migration and invasion of GC cells. (a and b) MGC-803-mock, MGC-803-long TIPE3 and MGC-803-short TIPE3 cells were used for migration and invasion assays. Statistical analysis was performed by counting the number of cells passing through the membrane and Matrigel. (c–g) The effect of TIPE3 isoforms on the expression of MMP2 and MMP9 was determined by RT-qPCR and western blotting. All data are shown as the means ± SD of three independent experiments with similar results. (*P < 0.05, **P < 0.01 and ***P < 0.001 compared with mock; #P < 0.05, ##P < 0.01 and ###P < 0.001 compared with long TIPE3).

Article Snippet: Primary antibodies against β-actin (#4970), 3flag (#14793), PI3K (#4257), p-PI3K (#13857), Akt (#4691), p-Akt (#4060), MMP2 (#4200), MMP9 (#2270) and anti-rabbit IgG (H + L) (DyLightTM 800 4X Conjugate) (#5151) were obtained from Cell Signaling Technology (Danvers).

Techniques: Migration, Membrane, Expressing, Quantitative RT-PCR, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Effects of the long and short isoforms of TIPE3 on the growth and metastasis of gastric cancer.

doi: 10.1016/j.biopha.2020.109853

Figure Lengend Snippet: Fig. 5. Long TIPE3 and short TIPE3 promoted the proliferation, migration and invasion of GC cells by activating the PI3K/Akt pathway. (a-–c) The phosphorylation levels of p-PI3K and p-Akt were significantly increased in cells overexpressing long TIPE3 and short TIPE3. Meanwhile, the activation of short TIPE3 was stronger. (a) Inhibiting the PI3K/Akt pathway by LY294002 (15 μmol/L) could obviously decrease the upregulated phosphorylation levels of p-PI3K and p-Akt. (d-–g) Inhibiting the PI3K/Akt pathway by LY294002 could block the upregulated proliferation, colony formation, migration and invasion abilities induced by long TIPE3 and short TIPE3 overexpression. (h-–j) The mRNA and protein levels of MMP2 and MMP9 were significantly decreased. All data are shown as the means ± SD from experiments repeated three independent times with similar results. (*P < 0.05, **P < 0.01 and ***P < 0.001 compared with mock; #P < 0.05, ##P < 0.01 and ###P < 0.001 compared with long TIPE3).

Article Snippet: Primary antibodies against β-actin (#4970), 3flag (#14793), PI3K (#4257), p-PI3K (#13857), Akt (#4691), p-Akt (#4060), MMP2 (#4200), MMP9 (#2270) and anti-rabbit IgG (H + L) (DyLightTM 800 4X Conjugate) (#5151) were obtained from Cell Signaling Technology (Danvers).

Techniques: Migration, Phospho-proteomics, Activation Assay, Blocking Assay, Over Expression